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IFIT(Interferon induced proteins with tetratricopeptide repeats)家族基因是一组较早发现的干扰素刺激基因,它在抗病毒和免疫调节中发挥了重要作用。为研究IFIT家族基因抑制A型流感病毒复制的机理,利用高通量RNA深度测序(RNA-Seq)技术发现A型流感病毒A/WSN/33(WSN)毒株感染293T细胞后,IFIT家族基因均出现明显上调。同时发现在IFIT2、IFIT3过表达后,流感病毒的复制和转录均有明显下调,并对v RNP聚合酶活性具有剂量依赖型的抑制作用。进一步研究证明在感染IFIT2、IFIT3编码蛋白与流感病毒非结构蛋白(NS1)存在细胞内共定位,证明二者存在相互作用的可能。综上所述,IFIT家族基因可以抑制A型流感病毒的复制和转录,有助于进一步阐明宿主因子对流感病毒感染的调节机制。
The family of Interferon induced proteins with tetratricopeptide repeats (IFITs) is a group of earlier found interferon stimulating genes that play an important role in antiviral and immunomodulatory effects. In order to study the mechanism of IFIT family gene inhibiting influenza A virus replication, high-throughput RNA sequencing (RNA-Seq) was used to detect the infection of 293T cells with influenza virus A / WSN / 33 (WSN) Significantly increased. It was also found that IFIT2 and IFIT3 overexpression significantly down-regulated the replication and transcription of influenza virus and inhibited the activity of v RNP polymerase in a dose-dependent manner. Further studies demonstrated that IFIT3 interacted intracellularly with the IFIT3-encoded protein and the nonstructural protein (NS1) of influenza virus in IFIT2 infection, demonstrating the possibility of interaction between the two. In summary, the IFIT family of genes can inhibit the replication and transcription of influenza A virus, help to further clarify the regulatory mechanism of host factors on influenza virus infection.