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为了探讨邻苯二甲酸二(2-乙基己基)酯(DEHP)对人绒毛外滋养层细胞HTR-8/Svneo侵袭和迁移的影响及机制,分别采用MTT法和流式细胞术确定DEHP的作用浓度,Transwell小室检测DEHP对细胞侵袭和迁移能力的影响,Rea;-time PCR和Western blot检测侵袭与迁移相关因子表达的变化。研究结果显示:100μmol/L及以上浓度的DEHP可促进HTR-8/Svneo细胞侵袭、迁移及MMP-2、MMP-9的表达,并上调p38、JNK的磷酸化,而DEHP的作用受p38与JNK的抑制剂拮抗。由此证明DEHP可通过p38、JNK信号通路上调MMP-2、MMP-9的表达,进而促进HTR-8/Svneo细胞侵袭和迁移。
To investigate the effect and mechanism of DEHP on the invasion and migration of HTR-8 / Svneo cells in human villi and trophoblast cells, MTT and flow cytometry were used to determine the effect of DEHP The effects of DEHP on the invasion and migration of cells were detected by Transwell chamber, Rea. The expressions of invasion and migration related factors were detected by time PCR and Western blot. The results showed that DEHP at a concentration of 100μmol / L and above promoted the invasion, migration, MMP-2 and MMP-9 expression and up-regulated the phosphorylation of p38 and JNK in HTR-8 / Svneo cells. Inhibitors of JNK antagonize. Thus, DEHP can up-regulate the expression of MMP-2 and MMP-9 through the p38 and JNK signaling pathways and further promote the invasion and migration of HTR-8 / Svneo cells.