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黄曲霉毒素B_1对大鼠、小鼠、豚鼠、猴及其他动物的毒理作用和致癌作用早已阐明,其在人体内的代谢,只有借助于尿、粪及尸体解剖材料的分析。本文作者报导了两例人体肝组织在离体保温条件下代谢黄曲霉毒素的结果。将标记的黄曲霉毒素B_1(~(14)C-AFB_1)加入活体标本和尸体解剖所得人肝组织匀浆上清液,并和NADPH发生系统(包括NADP~+,葡萄糖-6-磷酸和葡萄糖-6-磷酸脱氢酶)在37℃-併盢?.5小时,然后经提纯及薄层层析法检定其氯仿抽提的代谢物,再用液体闪烁计数法予以定量。代谢
The toxicological and carcinogenic effects of aflatoxin B1 on rats, mice, guinea pigs, monkeys and other animals have long been elucidated that its metabolism in the human body can only be analyzed by means of urine, feces and autopsy materials. The authors report the results of two human liver tissues metabolizing aflatoxins under ex vivo incubation conditions. The labeled aflatoxin B_1 (~ (14) C-AFB_1) was added into the biopsy of human liver homogenates and the supernatant of the liver homogenates obtained from the autopsy and incubated with the NADPH generating system (including NADP +, glucose-6-phosphate and glucose -6-phosphate dehydrogenase) at 37 ℃ - and 盢? .5 hours, and then purified by thin layer chromatography and its chloroform extract of the metabolites, and then quantified by liquid scintillation counting. metabolism