论文部分内容阅读
文献中在体神经放电的慢记录方法报道很少.为了解~(60)Co照射后迷走神经的兴奋性变化与照射后胃运动抑制的关系,建立了大鼠埋藏电极慢记录膈下迷走神经传出放电的方法.具体方法如下:记录电极,取直径0.4mm、长4mm的铂丝,一端与长约18cm的塑料绝缘的细导线(截面积为0.035mm)焊接,另一端弯成圆环形,但不封口,环的内径略粗于神经.做同样的二个环形电极.电极套管,套管用聚乙烯管制做,管的直径为2mm,长6mm.电极套管起固定电极并使之与周围组织绝缘的作用.电极的埋藏,实验动物为大白鼠,体重150～200g.用1%戊巴比妥钠(30mg/kg)腹腔注射麻醉.剪去腹部的毛,在无菌条件下沿中线作一切口.切开腹腔后将胃向下拉,使食道下段充分暴露.用细玻璃针分离食道腹侧迷走神经,分离长度约1cm.将两个电极环套在神经上,封闭环口.从套管开口处将套管套在连有电极的神经上.电极引线自套管的两个小缺口引出.用丝线结扎套管的三个部位(引线两边及中间),使套管的纵向切口紧闭,紧
In the literature, there are few reports of slow recordings on somatic discharge in order to understand the relationship between excitatory changes of vagal nerves and gastric motility after irradiation in order to find out the slow recording of subarachnoid vagus nerve Discharge method, the specific method is as follows: recording electrode, take the diameter of 0.4mm, 4mm long platinum wire, one end and a plastic insulated thin wire about 18cm (cross-sectional area of 0.035mm) welding, the other end is bent into a circular ring, But not sealing, the inner diameter of the ring is slightly thicker than the nerves. Do the same two ring electrodes. Electrode cannula, cannula made of polyethylene pipe, tube diameter of 2mm, length 6mm. The role of the surrounding tissue insulation electrode buried, the experimental animals for rats, weighing 150 ~ 200g with 1% sodium pentobarbital (30mg / kg) intraperitoneal injection anesthesia. Abdomen hair cut under sterile conditions along the The middle of the line for all incision. Open the abdominal cavity after the stomach down, so that full esophageal exposure. Thin glass needle separation of the ventral vagus nerve, the separation of about 1cm in length. Place the cannula over the nerve with the electrode open at the opening of the cannula Two small gap from the sleeve lead. Ligated with silk thread three sleeve portions (on both sides and the intermediate wire), that the longitudinal slit of the sleeve closed, tight