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Background/Aims The development of human malignancies can be attributed to abe rrant regulation of intracellular signal transduction pathways. In the current s tudy, we aimed to evaluate focal adhesion kinase (FAK), a non-receptor tyrosine kinase, expression in hepatocellular carcinoma (HCC), and to explore the progno stic significance of FAK. Methods We investigated FAK mRNA expression in 60 HCC specimens using quantitative real-time reverse transcription polymerase chain r eaction analysis, and the correlation between FAK expression and clinicopatholog ic parameters. FAK protein expression was examined using Western blot analysis a nd an immunohistochemical study. Results We found that FAK mRNA was overexpresse d in HCCs compared with the corresponding noncancerous liver tissues (P=0.0008). The FAK overexpression correlated significantly with tumor size (P=0.034) and s erum AFP level (P=0.030). Univariate and multivariate analyses revealed that FAK mRNA expression was an independent prognostic factor for disease-free (risk ra tio 3.83; P=0.024)and overall (risk ratio 7.14; P=0.015) survival. Besides, we c onfirmed immunohistochemically that the FAK protein was detectable in cancer cel ls despite non-expression in corresponding non-cancerous tissues. Conclusions Our results suggest that FAK mRNA expression has prognostic significance for the survival of patients with HCC.
Background / Aims The development of human malignancies can be attributed to abe rrant regulation of intracellular signal transduction pathways. In the current study, we aimed to assess focal adhesion kinase (FAK), a non-receptor tyrosine kinase, expression in hepatocellular carcinoma HCC), and to explore the prognostic significance of FAK. Methods We investigated FAK mRNA expression in 60 HCC specimens using quantitative real-time reverse transcription polymerase chain reaction analysis, and the correlation between FAK expression and clinicopathological parameters. FAK protein expression was examined using Western blot analysis a nd an immunohistochemical study. Results We found that FAK mRNA was overexpresse d in HCCs compared with the corresponding noncancerous liver tissues (P = 0.0008). The FAK overexpression correlated significantly with tumor size (P = 0.034) and s erum AFP level (P = 0.030). Univariate and multivariate analyzes revealed that FAK mRNA expression was an independent prognostic factor for disease-free (hazard ratio 3.83; P = 0.024) and overall corresponding non-cancerous tissues. Conclusions Our results suggest that FAK mRNA expression has prognostic significance for the survival of patients with HCC.