目的 :探讨实验室条件下人体角朊细胞的培养技术 ,观察角朊细胞培养生长过程。方法 :采用改良的有血清培养技术进行人体角朊细胞的培养。结果 :培养的角朊细胞 5天融合达 70 % ,9天达 90 % ,1 1天左右完全融合 ,细胞分裂指数高峰在 9天左右 ,可达 1 1 %。结论 :利用改良的有血清培养技术体外培养角朊细胞生长好 ,9～ 1 1天即可形成膜片 ,可达到移植条件 ,对临床上救治大面积烧伤病人具有重要意义 Objective: To investigate the culture of human keratinocytes under laboratory conditions and to observe the growth of keratinocytes. Methods: The cultured human keratinocytes were cultured with modified serum culture technique. Results: The cultured keratinocytes reached 70% in 5 days, 90% in 9 days and completely fused in 11 days. The peak of cell division index was about 9 days, up to 11%. CONCLUSION: Keratinocytes cultured in vitro with modified serum-cultured technology have good growth and can form membrane from 9 to 11 days, which can reach the condition of transplantation and is of great significance for the treatment of patients with extensive burns in clinic