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目的研究有机物对二氧化氯灭活脊髓灰质炎病毒的影响。方法采用悬液定量灭活试验方法和细胞感染技术,对病毒悬液中小牛血清白蛋白影响二氧化氯灭活脊髓灰质炎病毒的效果进行了观察。结果用含量30 mg/L二氧化氯作用15 min,对含高浓度小牛血清白蛋白悬液中脊髓灰质炎病毒灭活对数值仅为0.22,对含低浓度小牛血清白蛋白悬液中脊髓灰质炎病毒灭活对数值为3.28。用含量为40 mg/L二氧化氯作用15 min,对含高浓度小牛血清白蛋白悬液中脊髓灰质炎病毒灭活对数值均<4.00,对含低浓度小牛血清白蛋白悬液中脊髓灰质炎病毒灭活对数值均>4.00。结论病毒悬液内含小牛血清白蛋白明显影响二氧化氯对病毒灭活效果,且随其浓度增加影响程度增加。
Objective To study the effect of organics on chlorine dioxide inactivated poliovirus. Methods The effect of chlorine dioxide inactivation of poliovirus on the suspension of bovine serum albumin (BSA) in the virus suspension was observed by quantitative inactivation test and cell infection technique. Results With a concentration of 30 mg / L of chlorine dioxide for 15 min, the logarithm value of inactivation of poliovirus in suspension containing high concentration of bovine serum albumin was only 0.22, and in the suspension containing low concentration of bovine serum albumin The inactivated log of polio virus was 3.28. With 40 mg / L chlorine dioxide for 15 min, the inactivation logarithm of poliovirus in suspensions containing high concentration of bovine serum albumin was <4.00, and in the suspension containing low concentration of bovine serum albumin The inactivated logarithm of poliovirus was> 4.00. Conclusion The virus suspension containing bovine serum albumin significantly affects the inactivation effect of chlorine dioxide on the virus, and increases with the increase of its concentration.