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研究黄芪对双歧杆菌生长和活菌数的影响,并探索黄芪联合双歧杆菌对小鼠黑色素瘤模型的作用。在不同培养条件下,利用全自动生长曲线分析仪实时测定光密度值A600的变化,与对照组相比,比较黄芪对双歧杆菌活菌数的影响。建立小鼠黑色素瘤动物模型,测定肿瘤体积大小变化和小鼠生存率,研究黄芪联合双歧杆菌对荷瘤小鼠的抑瘤效果。结果表明,与不加药空白对照相比,黄芪组不仅能提高300μL小体系下双歧杆菌的生长,而且当体系扩大至1 m L后结果相同,说明不同培养时间和不同培养体系下,黄芪都具有促进双歧杆菌生长的作用。通过进一步对双歧杆菌生长曲线的分析发现,黄芪组较对照组对双歧杆菌的生长速率虽没有显著影响,但是能显著延长双歧杆菌到达平台期的时间,从而提高菌量。同时,黄芪对双歧杆菌生长的促进作用具有浓度依赖关系,浓度越高,促进效果越好,以1 g/m L浓度的效果最佳。在动物实验中,与NS组相比,黄芪与双歧杆菌联用组抑瘤效果最显著,其肿瘤体积明显小于其它各组(P<0.05),同时对荷瘤小鼠的生存率也明显延长(P<0.05)。黄芪在体外可促进双歧杆菌的生长,其抗肿瘤作用可能与提高双歧杆菌的数量相关,该特性具有潜在的应用价值。
The effects of Astragalus membranaceus on the growth and viable count of Bifidobacterium were studied, and the effect of Astragalus membranaceus and Bifidobacterium on mouse melanoma model was explored. Under different culture conditions, the change of optical density value A600 was measured in real time using an automatic growth curve analyzer, and the effect of Astragalus membranaceus on viable bifidobacteria was compared with that of the control group. The animal model of mouse melanoma was established, the change of tumor volume and the survival rate of mice were determined, and the anti-tumor effect of Astragalus combined with Bifidobacterium on tumor-bearing mice was studied. The results showed that astragalus group not only increased the growth of Bifidobacterium under 300μL small system compared with the blank control group, but also showed the same result when the system expanded to 1m L, which indicated that Astragalus mongholicus under the different culture time and culture system Have the role of promoting the growth of Bifidobacteria. By further analysis of the growth curve of bifidobacteria, it was found that the growth rate of Bifidobacterium was not significantly affected by Astragalus membranaceus compared with the control group, but it could significantly prolong the plateau period of Bifidobacterium and increase the amount of bacteria. At the same time, Astragalus had a concentration-dependent relationship with the promotion of Bifidobacterium growth. The higher the concentration was, the better the promotion effect was. The best concentration was 1 g / mL. Compared with NS group, Astragalus and Bifidobacterium group had the most significant antitumor effect in animal experiment, the tumor volume was obviously smaller than other groups (P <0.05), and the survival rate of tumor-bearing mice was also obvious Extended (P <0.05). Astragalus can promote the growth of Bifidobacterium in vitro, and its anti-tumor effect may be related to increasing the quantity of Bifidobacterium, which has potential application value.