论文部分内容阅读
为了更全面地了解戊型肝炎病毒（ＨＥＶ）的感染过程，从ＨＥＶ开放读框１（ＯＲＦ１）区设计两对寡核苷酸引物，建立逆转录－套式聚合酶链反应（ＲＴ－ｎＰＣＲ），对６２例戊型肝炎患者发病后５３１份血清进行ＨＥＶＲＮＡ检测。结果显示，阳性患者占７１％。全程随访３２例患者２８８份系列血清，发现阳性２４例（７５％），其阳性率随病程延长而下降，平均持续时间为发病后２０．６天。观察４４例血清ＨＥＶＲＮＡ阳性患者，有３６例（８１．８％）在血清转氨酶和总胆红素水平开始回落时仍呈阳性；３９例（８８．６％）同时检测到抗－ＨＥＶ。ＨＥＶ血症的阳性率及阳性持续时间与血清转氨酶、总胆红素及抗体应答水平无直接相关
To understand more about the HEV infection process, two pairs of oligonucleotide primers were designed from HEV open reading frame 1 (ORF1) region to establish a reverse transcription-nested polymerase chain reaction (RT-nPCR) , HEV RNA was detected in 531 sera from 62 hepatitis E patients. The results showed that positive patients accounted for 71%. A total of 288 sera from 32 patients were followed up, 24 (75%) positive were found. The positive rate decreased with the prolongation of the course of disease. The average duration was 20.6 days after onset. Thirty-six patients (81.8%) with positive HEV RNA were still positive at the onset of serum aminotransferase and total bilirubin levels, and 39 (88.6%) with anti-HEV. There was no direct correlation between the positive rate of HEV seropositivity and the positive duration of serum ALT, total bilirubin and antibody response