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目的探讨一种更准确的布鲁菌抗体确认法,为布氏疫情防控提供及时、科学的判断依据。方法先用虎红平板法对采集的血清样本进行初筛,再用试管凝集法及ELISA法对初筛阳性样本进行检测,并对两种方法检测结果不一致的样本用PCR法进行确认,最后对相关结果进行分析与评价。结果分别从526份羊与104份人血清中初筛得到阳性样本170、15份。同时用SAT与ELISA法对阳性样本进行检测,其中18份样本检测结果不一致,两种方法结果符合率为90.3%。18份有异议的样本经PCR法验证,结果显示ELISA准确率为100%。结论初步认为ELISA法可替代SAT作为一种更准确、更便捷的布鲁菌抗体确认方法。
Objective To investigate a more accurate confirmation method of Brucella antibody to provide a timely and scientific basis for the prevention and control of brucellosis. Methods Serum samples were preliminarily screened by tiger red plate method. Then positive samples were detected by tube agglutination and ELISA, and samples inconsistent with the two methods were confirmed by PCR method. Finally, Related results for analysis and evaluation. Results A total of 170,15 positive samples were obtained from 526 sheep and 104 human serum respectively. At the same time, the positive samples were detected by SAT and ELISA. The test results of 18 samples were inconsistent. The coincidence rate of the two methods was 90.3%. Eighteen objectionable samples were validated by PCR and showed that the ELISA accuracy was 100%. Conclusion It is preliminarily believed that ELISA can replace SAT as a more accurate and convenient method for the confirmation of Brucella antibodies.