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以大豆为外源DNA供体 ,用浸种及幼苗期浇灌法和花粉管通道法直接将大豆总DNA导入受体水稻 ,经常规栽培获得水稻后代种子。采用微量凯氏定氮法和氨基酸自动分析仪进行水稻后代种子糙米的粗蛋白含量和氨基酸含量的测定。结果显示 :三组经大豆DNA溶液处理获得的水稻后代种子糙米粗蛋白平均含量分别为 1 6 .42 %、1 6 .80 %和 1 9.87% ,与对照组相比有明显提高 ,统计分析都达到极显著的差异 (P <0 .0 1 ) ;在氨基酸含量测定中 ,有些材料的总氨基酸 (除色氨酸以外 )含量高达 1 7.2 0 %、1 6 .86 %和 1 6 .0 9% ,其中赖氨酸的含量分别为 0 .6 0 %、0 .6 0 %和 0 .5 7% ,与对照组相比也有明显提高 ,统计分析差异也都达到极显著水平(P <0 .0 1 )。本试验结果充分说明 ,利用大豆总DNA的导入方法有可能达到迅速有效地提高稻米蛋白质及赖氨酸含量的目的。
Soybean as an exogenous DNA donor, with soaking and seedling watering method and pollen tube pathway directly to the total soybean DNA introduced into the recipient of rice, the conventional cultivation of rice progeny seeds. Micro-Kjeldahl method and automatic amino acid analyzer were used to determine the crude protein content and amino acid content of rice progeny seed brown rice. The results showed that the crude protein content of the brown rice obtained from soybean DNA solution treatment was 16.42%, 16.8% and 1 9.87%, respectively, which was significantly higher than that of the control group (P <0.01). The contents of total amino acids (except tryptophan) in some amino acids were as high as 1 7.2%, 16.86% and 16.0% %, In which the contents of lysine were 0.6%, 0.6% and 0.57%, respectively, which were significantly higher than that of the control group (P <0.05) .0 1). The results of this experiment fully demonstrate that the introduction of soybean total DNA may achieve the purpose of rapidly and effectively increasing the contents of rice protein and lysine.