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提出一种基于模板匹配的ColorChecker定位分割算法，该算法不需要使用颜色值等信息，避免拍摄环境差异造成的定位失败。经过实验确定参数后，成功用于1400例舌部图像中ColorChecker的定位。经过改进后，该算法可以进一步提高效率，并用于进一步的开发。“,”Proposes a positioning and segmentation algorithm for ColorChecker based on template matching, this algorithm avoids using color value because its difference will lead to unsuccessfully positioning. After tuning parameters, this algorithm segments colorcheckers in 1400 tongue images successfully. Modifications for this algorithm will lead more efficient and can be used in other developments.