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目的探讨细胞增殖活性与成人型喉乳头状瘤生物学行为的关系。方法４２例手术及活检标本，分为四组：正常喉粘膜组（ｎ＝５）、喉乳头状瘤组（ｎ＝２１）、喉乳头状瘤伴不典型增生组（ｎ＝９）及喉乳头状瘤癌变组（ｎ＝７），应用抗增殖细胞核抗原（抗ＰＣＮＡ）单克隆抗体ＰＣ１０，采用ＡＢＣ免疫组织化学染色技术，评价其细胞增殖活性。结果经ＰＣＮＡ阳性反应计算出的ＰＣＮＡ增殖指数分别为３．８５０±０．６３６％、８．５８８±１．４０９％、１１．２２０±１．６０２％、１６．８１７±２．８９９％，各组间经统计学分析有差异（Ｐ＜０．０５）。结论ＰＣＮＡ表达可作为喉部良、恶性病变的鉴别，检测癌前病变及观察肿瘤生物学行为较好的参考指标。
Objective To investigate the relationship between cell proliferation activity and biological behavior of adult laryngeal papilloma. Methods 42 cases of surgical and biopsy specimens were divided into four groups: normal laryngeal mucosa (n = 5), laryngeal papilloma (n = 21), laryngeal papilloma with dysplasia (n = 9) (N = 7). The anti-proliferating cell nuclear antigen (PCNA) monoclonal antibody PC10 was used to evaluate the cell proliferative activity by ABC immunohistochemical staining. Results PCNA proliferation index calculated by PCNA positive reaction were 3.850 ± 0.636%, 8.588 ± 1.409%, 11.220 ± 1.602% and 16.817 ± 2.899% There were differences between groups by statistical analysis (P <0.05). Conclusion The expression of PCNA can be used as a differential diagnosis of benign and malignant lesions of the larynx. The detection of precancerous lesions and the observation of good biological behavior of the reference index.