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1996～ 1998年测定江苏、湖北、浙江、安徽、陕西、黑龙江部分地区油菜菌核病菌对多菌灵的抗药性。在被测的 1890份样本中已有 746株产生抗药性 ,抗药性菌株检出率达 39.5 % ,表明油菜菌核病菌已在江苏、湖北、安徽省部分地区内对多菌灵产生抗药性 ,其中 1997年江苏通州某些田块的抗性比例达 10 0 %。离体和活体敏感性测定结果表明 ,抗性菌株均为高抗菌株 ,其 EC5 0 >10 0μg/ml、MIC>10 0 0μg/m l。 3年监测结果显示 ,油菜菌核病菌对多菌灵产生抗性的范围已逐年扩大 ,抗性比例升高。抗多菌灵菌株对苯菌灵、噻菌灵和甲基硫菌灵表现为正交互抗性 ,对乙霉威 (MDPC)表现为负交互抗性 ;三唑类和二甲酰亚胺类杀菌剂对多菌灵的抗性和敏感菌株均表现敏感。
From 1996 to 1998, the resistance to carbendazim in Sclerotinia sclerotiorum in Jiangsu, Hubei, Zhejiang, Anhui, Shaanxi and Heilongjiang provinces was determined. Among 1890 samples tested, 746 strains were found to be drug-resistant, and the detection rate of drug-resistant strains was 39.5%, indicating that B. rapa was already resistant to carbendazim in Jiangsu, Hubei and Anhui provinces, Among them, the resistance rate of some plots in Tongzhou, Jiangsu province in 1997 reached 100%. The results of in vitro and in vivo susceptibility assays showed that all the resistant strains were highly resistant to EC50> 10 0μg / ml and MIC> 10 0μg / ml. The 3-year monitoring results showed that the range of resistance to carbendazim in Sclerotinia sclerotiorum has been expanded year by year, and the proportion of resistance increased. The anti-carbendazim strains exhibited orthogonal cross-resistance to benomyl, thiabendazole and thiophanate-methyl, negative cross-resistance to dimethyldiaminide (MDPC). Triazoles and dicarboximides Bactericides are sensitive to carbendazim resistance and susceptible strains.