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目的:探讨血管生成拟态(VM)和低氧诱导因子-1α(HIF-1α)在食管鳞癌组织中的表达及意义。方法:收集临床病理资料完整的食管鳞癌组织170例,进行PAS及CD34双重染色,观察食管鳞癌中是否存在VM;免疫组化SP法检测HIF-1α蛋白的表达,并计数微血管密度(MVD)。结果:在食管鳞癌组织中VM和HIF-1α表达阳性率分别为12.4%(21/170)、64.1%(109/170),显著高于正常食管黏膜组织,P<0.01。VM在低分化食管鳞癌组织中(25.9%)高于高-中分化组(9.8%),与TNM分期、淋巴结转移和MVD有关,P<0.05。HIF-1α的表达也与TNM分期、淋巴结转移及MVD有关,P<0.05;VM与HIF-1α蛋白表达相关,r=0.206,P<0.05。结论:在食管鳞癌组织中存在VM,HIF-1α高表达可能促进VM。VM与HIF-1α高表达可能是食管鳞癌浸润、转移重要生物学标志。VM与HIF-1α联合检测对食管鳞癌的进展及预后判断有重要意义。
Objective: To investigate the expression and significance of angiogenic mimicry (VM) and hypoxia inducible factor-1α (HIF-1α) in esophageal squamous cell carcinoma. Methods: A total of 170 patients with esophageal squamous cell carcinoma of the esophagus were enrolled in this study. The PAS and CD34 double staining was performed to detect the presence or absence of VM in esophageal squamous cell carcinoma. The expression of HIF-1α protein was detected by immunohistochemical SP method. The microvessel density ). Results: The positive rates of VM and HIF-1α in esophageal squamous cell carcinoma were 12.4% (21/170) and 64.1% (109/170) respectively, which were significantly higher than those in normal esophageal mucosa (P <0.01). VM was higher in poorly differentiated esophageal squamous cell carcinoma (25.9%) than in high-moderately differentiated group (9.8%), which was associated with TNM staging, lymph node metastasis and MVD, P <0.05. The expression of HIF-1α was also correlated with TNM stage, lymph node metastasis and MVD (P <0.05). VM was correlated with HIF-1α protein expression, r = 0.206, P <0.05. Conclusions: VM exists in esophageal squamous cell carcinoma, and high expression of HIF-1α may promote VM. The high expression of VM and HIF-1α may be an important biological marker for the invasion and metastasis of esophageal squamous cell carcinoma. Detection of VM and HIF-1α in esophageal squamous cell carcinoma and prognosis of great significance.