〔抗原制备〕以BSA为载体蛋白、水溶性碳二亚胺为联结剂,制备结合抗原PGE_2—BSA, PGF_(2_a)—BSA(牛血清白蛋白);每个BSA分子约可联结PG分子17个左右。〔抗体制备〕第1周1毫克PG—BSA溶于0.5毫升生理盐水与0.5毫升福氏完全佐剂(5毫克活卡/毫升)充分乳化后,于动物背部作皮内多点注射,进行基础免疫。第2～4周每周免疫一次。除以福氏不完全佐剂外,其他方法及剂量同第一周。隔一月后,每月静脉加皮下注射1毫克PG—BSA/1毫升生理盐水,加强免疫一次。 [Preparation of Antigen] The binding antigen PGE_2-BSA and PGF_ (2_a) -BSA (bovine serum albumin) were prepared using BSA as a carrier protein and a water-soluble carbodiimide as a binding agent; each BSA molecule can bind to about PG molecule 17 A or so. [Preparation of Antibody] The first week, 1 mg PG-BSA was thoroughly emulsified in 0.5 ml normal saline and 0.5 ml Freund’s complete adjuvant (5 mg Lactam / ml), and injected into the back of the animal for multiple injections Immune. Weeks 2 to 4 are immunized weekly. Divided by incomplete incomplete Freund’s adjuvant, the other methods and doses the same week. Every other month, a monthly intravenous injection of 1 mg of PG-BSA / 1 ml of normal saline, booster immunization.