论文部分内容阅读
目的：探讨肝部分切除后肝再生过程中线粒体能量代谢的调控。方法：用雄性Ｗｉｓｔａｒ大鼠施行肝部分切除复制肝再生模型，肝部分切除后分别观察０５、１、２、４和７ｄ５个肝再生组以及５个相应的对照组，差速离心法分离肝线粒体并用氧电极极谱法测定其氧化磷酸化活性。结果：肝部分切除后肝再生过程中线粒体呼吸控制率（ＲＣＲ）明显高于相应对照组，其中肝部分切除后０５ｄ和４ｄ为二个峰值，７ｄ时降至对照组水平，早期ＲＣＲ的升高主要是Ｒ３升高的所致，１ｄ后ＲＣＲ升高是Ｒ４下降所致。磷氧比值（Ｐ／Ｏ）的变化类似于ＲＣＲ。结论：肝部分切除后肝再生过程中线粒体通过氧化磷酸化偶联增强来适应肝再生的能量需求，这种增强机制很可能主要是通过降低线粒体内膜通透性实现的。
Objective: To investigate the regulation of mitochondrial energy metabolism during hepatic regeneration after partial hepatectomy. METHODS: Male Wistar rats were subjected to partial hepatectomy and resection of liver regeneration model. Five liver regeneration groups at 05, 1, 2, 4 and 7 days and 5 corresponding control groups were observed after partial hepatectomy. Differential centrifugation Liver mitochondria were assayed for their oxidative phosphorylation activity using oxygen electrode polarography. Results: Mitochondrial respiration rate (RCR) in liver regeneration was significantly higher than that of the corresponding control group after partial hepatectomy. There were two peaks at 05d and 4d after partial hepatectomy, and dropped to the control level on the 7th day. The rate of early RCR Rise is mainly due to the rise of R3, 1d after RCR rise is due to the decline of R4. The change in P / O ratio is similar to RCR. CONCLUSIONS: Mitochondria during liver regeneration after partial hepatectomy are augmented by oxidative phosphorylation to adapt to the energy requirement of liver regeneration, and this enhancement mechanism is likely to be mainly through the reduction of mitochondrial permeability.