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目的:观察宫颈细胞脱氧核糖核酸(DNA)定量分析及高危型人类乳头瘤病毒(HPV)检测在宫颈癌筛查中的应用,分析其临床价值。方法:选取重庆市江津区妇幼保健院2014年2月至2016年3月收治的146例无宫颈癌脱落细胞学禁忌症的患者。分别对这些患者实施DNA定量分析、高危型HPV检测,并根据DNA定量分析结果,将检测人员分成实验活检组和非活检组。结果:本次无宫颈癌脱落细胞学禁忌证146例患者的经过DNA定量分析、高危型HPV检测,按照宫颈活检病理结果为标准,计算出细胞DNA定量分析的灵敏度为89.6%,特异度为79.2%;高危型HPV检测的56例患者呈现阳性,22例呈现阴性;组织学阴性22例,阳性46例。高危型HPV检测的敏感性为95.7%,特异性为58.2%。常规细胞检测组敏感性为56.2%,特异性为43.6%。由此可见,DNA定量分析和高危型HPV检测与常规的细胞学相比,具有统计学意义(P<0.05)。结论:宫颈细胞DNA定量分析及高危型HPV检测在宫颈癌筛查中应用的可靠性较高,具有很高的临床研究价值。
OBJECTIVE: To observe the quantitative analysis of DNA in cervical cells and the use of high-risk human papillomavirus (HPV) in cervical cancer screening and analyze its clinical value. Methods: A total of 146 cases of contraindications to cervical cancer exfoliated cytology from February 2014 to March 2016 in Jiangjin District of Chongqing Municipality were selected. The DNA quantification and high-risk HPV tests were performed separately on these patients. According to the results of quantitative DNA analysis, the test personnel were divided into experimental biopsy group and non-biopsy group. Results: According to the biopsy results of cervical biopsies, 146 patients with cervical cytology without contraindication for cervical cancer were subjected to DNA quantification analysis and high-risk HPV test. The sensitivity and specificity of DNA quantitative analysis were 89.6% and 79.2 %; 56 cases of high-risk HPV test showed positive, 22 cases were negative; histological negative in 22 cases, 46 cases were positive. High-risk HPV test sensitivity was 95.7%, specificity was 58.2%. The sensitivity and specificity of routine cell test group were 56.2% and 43.6% respectively. Thus, DNA quantitative analysis and high-risk HPV testing compared with conventional cytology, with statistical significance (P <0.05). Conclusion: The quantitative determination of cervical cell DNA and high-risk HPV testing in the screening of cervical cancer is more reliable, with high clinical value.