精子线粒体DNA与男性生育能力密切相关,由于它缺少组蛋白和DNA结合蛋白的保护,易受活性氧的攻击,造成损伤,增加突变率,这被认为是引起男性不育的重要原因。精子mtDNA突变和数量的检测是评价男性生育力的重要方法。对精子mtDNA损伤的研究有重要意义。 Sperm mitochondrial DNA is closely related to male fertility. Due to its lack of protection of histones and DNA-binding proteins, mitochondrial sperm are susceptible to reactive oxygen species (ROS) attack, causing damage and increasing mutation rate, which is considered to be an important cause of male infertility. Sperm mtDNA mutation and the number of detection is an important method to evaluate male fertility. The study of sperm mtDNA damage is of great significance.