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目的分析新疆维吾尔族275例艾滋病病毒Ⅰ型(HIV-1)感染者和275名高危人群,对激活T细胞表达和分泌调节(RANTES)和基质细胞衍生因子(SDF1)-3′A等位基因的多态性和分布特点。方法利用聚合酶链反应-限制性内切酶片段长度多态性分析(PCR-RFLP)法,对维吾尔族高危人群和HIV-1感染者的各275份血液中的RANTES、SDF1-3′A等位基因进行检测。结果在275例高危人群中,RANTES、SDF1-3′A等位基因突变率分别为14.2%、19.7%,在275例HIV-1感染人群中,RANTES、SDF1-3′A等位基因突变率分别为19.6%、24.7%。χ2检验的结果提示,维吾尔族高危人群和HIV-1感染人群中,RANTES、SDF1-3′A基因多态性分布差异无统计学意义(P>0.05)。结论 RANTES、SDF1-3′A等位基因突变以及分布提示,在新疆维吾尔族人群HIV-1高危人群和感染人群中无差异性。
Objective To analyze 275 HIV-I and 275 high-risk Uygur nationalities in Xinjiang Uygur Autonomous Region (Uygur), and to investigate the role of RANTES and SDF1 -3’A alleles Polymorphism and distribution characteristics. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the expression of RANTES, SDF1-3’A in 275 blood samples of Uighur high risk population and HIV-1 infected population. Alleles are tested. Results The mutation rates of RANTES and SDF1-3’A alleles were respectively 14.2% and 19.7% in 275 high-risk population. Among 275 HIV-1 infected persons, the mutation rates of RANTES and SDF1-3’A alleles Respectively, 19.6%, 24.7%. The results of χ2 test indicated that there was no significant difference in the distribution of RANTES and SDF1-3’A polymorphism between Uighur high risk population and HIV-1 infected population (P> 0.05). Conclusions The mutations and distribution of RANTES and SDF1-3’A alleles suggest no difference in HIV-1-infected and HIV-1-infected Uighur populations.