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本研究比较了昆虫生长调节剂氟铃脲对棉铃虫生物测定的三种方法，并测定了其交互抗性。应用叶片残毒法和饲料混毒法测定结果表明，氟铃脲对１ 龄幼虫具有较高的毒力， Ｌ Ｃ５０ 分别为０ ．３５８ 和０ ．０１４ μｇ·ｍｌ－ １ 。饲料混毒法测定还表明，幼虫对氟铃脲的敏感性随着龄期的增加而降低，１ 龄幼虫的敏感性分别是２ ，３ ，４ ，５ ，６ 龄幼虫的２ ．６ ，２ ．７ ，３ ．４ ，３ ．８ ，６３ ．９ 倍。用点滴法测定，３ ，４ 龄幼虫对氟铃脲也具有较高的敏感性， Ｌ Ｄ５０ 分别为０ ．０５３ 和０ ．２７６ μｇ／ 头。比较三种生测方法，叶片残毒法快速、简易、可靠，故更适于作为昆虫生长调节剂对棉铃虫的抗性测定。叶片残毒法用１ 龄幼虫供试，接触处理４８ ｈ ，最佳观察时间为处理后５ ｄ 。交互抗性及增效剂 Ｐ Ｂ Ｏ 测定结果表明，对氰戍菊酯高抗的中国河南品系和印度品系对氟铃脲无交互抗性，其 Ｌ Ｃ５０ 或 Ｌ Ｄ５０ 与敏感品系比较相差仅分别为１ ．３４ ～２ ．１９ 倍和０ ．８４ ～１ ．７１ 倍。 Ｐ Ｂ Ｏ 对氟铃脲毒性的影响因剂量不同而异，低剂量时有增效作用，高剂量时反而有拮抗作用。 Ｐ Ｂ Ｏ５ μｇ／ 头对氟铃脲的增效比为１ ．６１ 。
In this study, we compared three methods for the bioassay of hexamers by hexaflumuron, an insect growth regulator, and determined their cross-resistance. The results of leaf residue remnant method and feed compound method showed that hexaflumuron had higher virulence to the first instar larvae with L C50 of 0. 358 and 0. 014 μg · ml-1. The feed poisoning assay also showed that the sensitivity of the larvae to hexaflumuron decreased with the increase of the age, the sensitivity of the first instar larvae was 2, 3, 4, 5 and 6 instar larvae respectively. 6, 2. 7, 3. 4, 3. 8, 63. 9 times. The results showed that the 3rd and 4th instar larvae had higher sensitivity to hexaflumuron with L D50 of 0. 053 and 0. 276 μg / head. Comparison of three methods of bioassay, leaf remnants toxicity fast, simple and reliable, it is more suitable as a insect growth regulator of cotton bollworm resistance determination. Leaf residue remnants of the first instar larvae test, contact treatment 48 h, the best observation time for 5 days after treatment. The cross resistance and synergist P B O assay results showed that Chinese Henan and India strains with high resistance to cypermethrin showed no cross-resistance to hexaflumuron, and the difference between L C50 or L D50 and susceptible strains was only 1 34 ~ 2. 19 times and 0. 84 ~ 1. 71 times. The effect of P B O on the toxicity of hexaflumuron varies with dose, with synergism at low doses and antagonism at higher doses. P B O 5 μg / head to hexaflumuron synergistic ratio of 1. 61.