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目的 应用Ｌａｎｇｅｎｄｏｒｆｆ 模型研究缺血预处理（ＩＰＣ） 对兔未成熟心肌缺血／ 再灌注损伤的影响并探讨其可能机制。方法 取３ ～４ 周未成熟离体兔心２４ 只，随机分为ＩＰＣ组和Ｃｏｎ 组，根据缺血前ＩＰＣ的次数将ＩＰＣ组分为ＩＰＣ１ 、ＩＰＣ２ 和ＩＰＣ３ ３ 个亚组，每组６ 只。ＩＰＣ１、ＩＰＣ２ 和ＩＰＣ３ ３ 个亚组的心肌常温平衡灌注２０ ｍｉｎ 后分别给予１、２、３ 个周期的５ ｍｉｎ 缺血和５ ｍｉｎ 再灌注，而Ｃｏｎ 组心肌在缺血前平衡灌注２０ ｍｉｎ 后续灌３０ ｍｉｎ。所有心肌均常温缺血４５ ｍｉｎ，再灌注３０ ｍｉｎ。于平衡末，缺血前，再灌注后即刻、３、５、１０、２０、３０ ｍｉｎ 时测定心脏的功能指标以及再灌注末心肌组织中ＡＴＰ、丙二醛（ＭＤＡ） 含量及超氧化物歧化酶（ＳＯＤ） 的活性。结果 ＩＰＣ１、ＩＰＣ２ 组的ＬＶＤＰ、＋ ｄｐ／ｄｔｍａｘ 及－ ｄｐ／ｄｔｍａｘ的恢复百分比显著高于Ｃｏｎ 组（ Ｐ ＜ ０．０５），ＩＰＣ２ 组＋ ｄｐ／ｄｔｍａｘ 显著高于ＩＰＣ３ 组（ Ｐ ＜ ０ ．０５） 。ＩＰＣ１、ＩＰＣ２ 组的ＬＶＥＤＰ值显著低于Ｃｏｎ 组（ Ｐ＜ ０．０５） 。ＩＰＣ可维持心肌组织中的ＡＴＰ在较高水平，以ＩＰＣ２ 组最
Objective To investigate the effect of ischemic preconditioning (IPC) on immature myocardial ischemia / reperfusion injury in rabbits and to explore its possible mechanism by Langendorff model. Methods Twenty-four immature rabbit hearts from 3 to 4 weeks were randomly divided into IPC group and Con group. IPC groups were divided into IPC1, IPC2 and IPC3 subgroups according to the number of IPC before ischemia, with 6 . IPC1, IPC2 and IPC3 three subgroups of normal myocardial perfusion after 20 min were given 1, 2 and 3 cycles of 5 min ischemia and 5 min reperfusion, and Con group myocardial perfusion before ischemia in 20 min continued Irrigation 30 min. All myocardial ischemia at room temperature 45 min, reperfusion 30 min. At the end of balance, before ischemia and immediately after reperfusion, the function indexes of heart, ATP, malondialdehyde (MDA) content and superoxide dismutase Enzyme (SOD) activity. Results The percentage of recovery of LVDP, + dp / dtmax and - dp / dtmax in IPC1 and IPC2 group was significantly higher than that in Con group (P <0.05), and IPC2 + dp / dtmax group was significantly higher than that in IPC3 group ). The LVEDP values in IPC1 and IPC2 group were significantly lower than those in Con group (P <0.05). IPC maintained a high level of ATP in myocardial tissue, most in the IPC2 group