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目的:评估纳米银颗粒的粘附对牙科合金的细胞毒性和抗菌性的影响,并初步探讨其作用机制。创新点:运用MTT法证实钴铬合金和纯钛对小鼠成骨前体细胞(MC3T3-E1)及大鼠骨髓间充质干细胞(BMSC)产生细胞毒性,粘附纳米银颗粒后细胞毒性有所降低。方法:将化学法制得的3种浓度的纳米银颗粒分别粘附于6种牙科合金表面,扫描电镜观察并确认纳米银的粘附情况。采用MTT法检测不同浓度纳米银颗粒的牙科合金对MC3T3-E1及BMSC的细胞毒性。评价3种浓度纳米银颗粒的钴铬合金和纯钛试件浸提液对金黄色葡萄球菌和变形链球菌的抗菌性。结论:牙科合金对MC3T3-E1和BMSC细胞具有较强的毒性,粘附纳米银颗粒后细胞毒性有所降低。3种浓度的纳米银颗粒细胞毒性之间无显著性差异,且这3种浓度纳米银颗粒粘附后对牙科合金的抗菌性无明显影响。
OBJECTIVE: To evaluate the effect of nano-silver particle adhesion on the cytotoxicity and antibacterial activity of dental alloys and to explore its mechanism. Innovative point: The cytotoxicity of cobalt-chromium alloy and pure titanium on mouse osteoblast precursor cells (MC3T3-E1) and rat bone marrow mesenchymal stem cells (BMSCs) was confirmed by MTT method. Cytotoxicity Reduced. METHODS: Three kinds of nano-sized silver particles were chemically adhered to the surface of six kinds of dental alloys. Scanning electron microscopy was used to observe the adhesion of nano-silver. The cytotoxicity of dental alloys with different concentrations of nano-silver particles on MC3T3-E1 and BMSCs was detected by MTT assay. To evaluate the antibacterial activity of extracts of cobalt-chromium alloy and pure titanium with three concentrations of nano-silver particles on Staphylococcus aureus and Streptococcus mutans. Conclusion: The dental alloys are highly toxic to MC3T3-E1 and BMSC cells, and the cytotoxicity is decreased after the nano-silver particles are adhered. There was no significant difference in the cytotoxicity between the three concentrations of nano-silver particles, and the adhesion of the three concentrations of nano-silver particles had no significant effect on the antibacterial activity of dental alloys.