在体外培养过程中，细胞的生长增殖除取决于细胞的特性、培养体系和条件以外，尚需有一定的细胞密度。单个细胞或密度极低的分散细胞很难存活和繁殖。为了促使刚刚融合克隆化的极少量细胞的存活和逐渐增殖，在培养板或培养瓶中加入能贴壁生长的其他活细胞后者称为“饲养细胞” In vitro culture, cell growth and proliferation in addition to depending on the cell characteristics, culture systems and conditions, the need for a certain cell density. Individual cells or very low density dispersed cells are difficult to survive and multiply. To promote the survival and proliferation of a very small number of cells that have just been fused and cloned, the addition of other viable cells adherent to the culture plate or flask is called “feeder cells”