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目的:观察胰岛素样生长因子I(IGF鄄I)对体外培养的大鼠成骨细胞的增殖、碱性磷酸酶(ALP)的合成和钙化结节形成的影响。方法:不同浓度IGF鄄I分别刺激培养的大鼠成骨细胞,采用噻唑蓝(MTT)法测定细胞增殖能力;采用对硝基酚磷酸盐法测定细胞裂解液中ALP活性;VonKossa染色测定钙化结节数目。结果:一定浓度IGF鄄I能明显增加大鼠成骨细胞数量(P<0.05),在0.1～100.0ng/ml这种作用与IGF鄄I的浓度呈正相关;经IGF鄄I刺激,大鼠成骨细胞ALP合成明显高于对照组(P<0.05);经IGF鄄I刺激,大鼠成骨细胞钙化结节数目明显高于对照组(P<0.001)。结论:IGF鄄I能增加体外培养的大鼠成骨细胞数量,促进成骨细胞ALP合成和钙化,提示IGF鄄I可能直接促进骨形成。
OBJECTIVE: To observe the effects of insulin-like growth factor I (IGF-I) on the proliferation of rat osteoblasts, the synthesis of alkaline phosphatase (ALP) and the formation of calcified nodules in vitro. METHODS: Cultured rat osteoblasts were stimulated with different concentrations of IGF-I, MTT assay was used to determine the cell proliferation. ALP activity in cell lysate was determined by p-nitrophenol phosphate assay. Calcification was measured by Von Kossa staining The number of sections. Results: IGF-I at a certain concentration significantly increased the number of osteoblasts in rats (P <0.05). The effect of IGF-I at a concentration of 0.1-100.0 ng / ml was positively correlated with the concentration of IGF-I. The ALP synthesis in osteocytes was significantly higher than that in the control group (P <0.05). The number of calcified nodules in osteoblasts stimulated by IGF-I was significantly higher than that in the control group (P <0.001). CONCLUSION: IGF-I can increase the number of osteoblasts cultured in vitro and promote ALP synthesis and calcification in osteoblasts, suggesting that IGF-I may directly promote bone formation.