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目的:观察脓毒症患者外周血Vδ1 T细胞数量和功能的改变,初步探讨Vδ1 T细胞在脓毒症发病中的作用。方法 :选取2013年12月—2014年12月在本院ICU入住的脓毒症患者40例及同期入院进行健康体检者(health control,HC)40例,清晨空腹抽取外周静脉血10 m L。采用流式细胞术测定其外周血Vδ1 T细胞比例及Vδ1 T细胞表面Foxp3表达情况,采用CFSE染色方法检测Vδ1 T细胞对CD4+T细胞增殖能力的影响,采用流式细胞术测定Vδ1 T细胞与CD4+T细胞共孵育后对CD4+T细胞分泌γ-干扰素(interferonγ,IFN-γ)及肿瘤坏死因子α(tumor necrosis factorα,TNF-α)能力的影响。结果 :脓毒症患者外周血Vδ1 T细胞数量(3.35±0.55)%与HC(1.04±0.22)%相比显著升高(P<0.01)。与HC外周血Vδ1 T细胞表面Foxp3表达水平(10.02±2.31)%相比,脓毒症患者外周血Vδ1 T细胞表面Foxp3表达水平(16.83±3.63)%显著升高(P<0.01)。HC外周血Vδ1 T细胞对CD4+T细胞增殖、分泌IFN-γ和TNF-κ的抑制百分率分别为(44.26±6.31)%、(34.84±4.83)%和(39.31±4.91)%,脓毒症患者外周血Vδ1 T细胞对CD4+T细胞增殖、分泌IFN-γ和TNF-κ的抑制百分率分别为(62.35±5.91)%、(50.37±4.77)%和(54.94±5.71)%。提示,脓毒症外周血Vδ1 T细胞较HC外周血Vδ1 T细胞具有更强的免疫抑制功能(P<0.01)。结论:脓毒症患者外周血Vδ1 T细胞免疫抑制功能增强,致使脓毒症患者免疫功能受到抑制,脓毒症患者免疫功能的这种改变可能与脓毒症的发生相关。
Objective: To observe the change of the number and function of Vδ1 T cells in peripheral blood of patients with sepsis and to explore the role of Vδ1 T cells in the pathogenesis of sepsis. Methods: Forty patients with sepsis who admitted to our ICU from December 2013 to December 2014 and 40 healthy controls (HC) admitted to hospital during the same period were enrolled. The percentage of Vδ1 T cells in peripheral blood and the expression of Foxp3 on Vδ1 T cells were measured by flow cytometry. The effect of Vδ1 T cells on the proliferation of CD4 + T cells was detected by CFSE staining. The Vδ1 T cells and Vδ1 T cells were detected by flow cytometry CD4 + T cells on the secretion of IFN-γ and TNF-α of CD4 + T cells after co-incubation with CD4 + T cells. Results: The number of Vδ1 T cells in peripheral blood of patients with sepsis (3.35 ± 0.55)% was significantly higher than that of HC (1.04 ± 0.22)% (P <0.01). Compared with the expression of Foxp3 on the surface of Vδ1 T cells in HC peripheral blood (10.02 ± 2.31)%, the expression level of Foxp3 on the surface of Vδ1 T cells in patients with sepsis (16.83 ± 3.63)% was significantly increased (P <0.01). The percentages of inhibition of CD4 + T cells proliferation and secretion of IFN-γ and TNF-κ by Vδ1 T cells from HC peripheral blood were (44.26 ± 6.31)%, (34.84 ± 4.83)% and (39.31 ± 4.91)%, respectively The percentages of Vδ1 T cells on CD4 + T cell proliferation, secretion of IFN-γ and TNF-κ in peripheral blood were (62.35 ± 5.91)%, (50.37 ± 4.77)% and (54.94 ± 5.71)%, respectively. These results suggest that Vδ1 T cells in peripheral blood of septic patients have more immunosuppressive function than Vδ1 T cells of HC peripheral blood (P <0.01). Conclusion: The immunosuppressive function of Vδ1 T cells in peripheral blood of septic patients is enhanced, resulting in the suppression of immune function in sepsis patients. The change of immune function in septic patients may be related to the occurrence of sepsis.