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目的观察不同浓度的人参皂苷Rg2(ginsenoside-Rg2)及其立体异构体[20(R)-Rg2和20(S)-Rg2]对氧糖剥离/再灌注(oxygen-glucose deprivation/reperfusion,OGD/R)细胞模型的保护作用,探讨其作用机制并比较天然型Rg2、20(R)-Rg2和20(S)-Rg2作用效果的差异性。方法将培养7天的皮层神经元分成5组:对照组、模型组、Rg2组、20(R)-Rg2组及20(S)-Rg2组,Rg2组、20(R)-Rg2组及20(S)-Rg2组分别加入浓度为20、40、80μmol/L的Rg2、20(R)-Rg2和20(S)-Rg2预先处理24 h,然后制备OGD/R模型;24 h后检测细胞存活率、凋亡因子Caspase-3的活性、胞内Ca2+浓度以及超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果与对照组比较,模型组细胞的存活率、SOD活力明显降低,Caspase-3的活性、Ca2+荧光灰度值及MDA含量明显升高,差异有统计学意义(P<0.05);与模型组比较,20、40、80μmol/L浓度时Rg2、20(R)-Rg2和20(S)-Rg2组细胞存活率、SOD活力均显著升高,Caspase-3活性、Ca2+荧光灰度值及MDA含量均显著降低(P<0.05);与20(S)-Rg2组比较,20、40、80μmol/L浓度时Rg2和20(R)-Rg2组的细胞存活率升高、MDA含量降低(P<0.05),80μmol/L浓度时20(R)-Rg2组及40、80μmol/L浓度时Rg2组的Caspase-3活性降低、SOD活力升高(P<0.05),40、80μmol/L浓度时Rg2和20(R)-Rg2组的Ca2+荧光灰度值降低(P<0.05);与20(R)-Rg2组比较,80μmol/L浓度时Rg2组的Ca2+荧光灰度值降低(P<0.05),40、80μmol/L浓度时Rg2组的SOD活力升高(P<0.05),20、40、80μmol/L浓度时Rg2组的MDA含量降低(P<0.05)。结论人参皂苷Rg2及其立体异构体预处理可以提高OGD/R条件下的细胞活力,其机制可能与提高细胞抗凋亡、抗氧化能力及减少Ca2+内流有关,并且20(R)-Rg2的作用效果优于20(S)-Rg2却低于天然型Rg2。
Objective To observe the effects of different concentrations of ginsenoside-Rg2 and its stereoisomers [20 (R) -Rg2 and 20 (S) -Rg2] on oxygen-glucose deprivation / reperfusion / R) cell model to explore its mechanism of action and to compare the effects of natural Rg2,20 (R) -Rg2 and 20 (S) -Rg2. Methods Cortical neurons cultured for 7 days were divided into 5 groups: control group, model group, Rg2 group, 20 (R) -Rg2 group and 20 (S) -Rg2 group, Rg2 group, 20 (R) -Rg2 group and 20 (S) -Rg2 group were pretreated with 20, 40 and 80μmol / L of Rg2,20 (R) -Rg2 and 20 (S) -Rg2 for 24 h, then OGD / R model was prepared. Survival rate, Caspase-3 activity, intracellular Ca2 + concentration, SOD and MDA contents. Results Compared with the control group, the survival rate, the activity of SOD, the activity of Caspase-3, the Ca2 + fluorescence intensity and the content of MDA in the model group were significantly increased (P <0.05). Compared with the model group Compared with the control group, the viability of cells, the activity of SOD and the activity of Caspase-3, the value of Ca2 + fluorescence intensity and the content of MDA in Rg2,20 (R) -Rg2 and 20 (S) -Rg2 groups were significantly increased at 20,40 and 80μmol / (P <0.05). Compared with 20 (S) -Rg2 group, the cell survival rate and the content of MDA in Rg2 and 20 (R) -Rg2 groups were significantly decreased at the concentrations of 20, 40 and 80μmol / L <0.05). Caspase-3 activity and SOD activity in Rg2 group were lower than those in 20 (R) -Rg2 group and 40,80μmol / L group at the concentration of 80μmol / L (P <0.05) Compared with 20 (R) -Rg2 group, the gray value of Ca2 + fluorescence in Rg2 and Rg2 decreased (P <0.05) (P <0.05) (P <0.05). At the concentrations of 40 and 80 μmol / L, the activity of SOD in Rg2 group increased (P <0.05). Conclusion Ginsenoside Rg2 and its stereoisomer pretreatment can increase the cell viability under OGD / R, which may be related to the enhancement of anti-apoptosis and anti-oxidation ability of cells and the decrease of Ca2 + influx, and that 20 (R) -Rg2 The effect is better than 20 (S) -Rg2 is lower than the natural type Rg2.