从150 mmol/L盐胁迫3 h和没有胁迫的耐盐水稻(Oryza sativa L.)品种特三矮2号叶片中提取mRNA,构建cDNA文库。通过示差筛选,得到3个盐胁迫应答cDNA克隆Ts1、Ts2和Ts3。Northern分析表明,3 h的盐胁迫可使Ts1、Ts2和Ts3的转录水平明显上升;3~24 h期间,Ts1和Ts2的转录水平继续上升,而Ts3的转录水平则下降。序列分析表明,这3个cDNA克隆与已知功能的基因没有同源性。利用特三矮2号的耐盐亲本ZYQ8和粳稻JX17组合构建了DH群体和分子标记连锁图谱,将Ts1、Ts2和Ts3分别定位在第1、3和7染色体上。值得注意的是,Ts1、Ts2和Ts3与用同一群体定位的主效和微效耐盐QTL位于同一或相邻区域。 The mRNA was extracted from the leaves of Te Sanzhao No.2, a salt-tolerant rice (Oryza sativa L.) under the stress of 150 mmol / L for 3 h and without stress. The cDNA library was constructed. Three salt stress response cDNA clones Ts1, Ts2 and Ts3 were obtained by differential screening. Northern analysis showed that the transcriptional levels of Ts1, Ts2 and Ts3 were significantly increased after 3 h of salt stress. The transcription levels of Ts1 and Ts2 continued to increase during 3-24 h while the levels of Ts3 decreased. Sequence analysis showed that these three cDNA clones have no homology with known function genes. The DH population and molecular marker linkage map were constructed using salt-tolerant parent ZYQ8 and japonica rice JX17, and Ts1, Ts2 and Ts3 were mapped on chromosomes 1, 3 and 7, respectively. It is noteworthy that Ts1, Ts2 and Ts3 are located in the same or adjacent regions as the major and minor salt-tolerant QTLs located in the same population.