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目的:研究不同保存条件下血清荧光定量HCV RNA监测结果的差异,对保存条件的影响效应进行分析。方法:将采集到的血清标本分为两组A组和B组,A组于零下二十摄氏度进行保存,持续七天,B组在A组的基础上于血清标本中加入RNA酶抑制剂于零下二十摄氏度进行保存,持续七天,对比两组血清标本检测结果。结果:两组血清标本检测结果差异不具备统计学意义,加入RNA酶抑制剂并不影响HCV RNA检测结果。结论:在实验研究的过程中,分别采集了20例患者的40份血清,均在零下二十摄氏度进行保存,其中一组加入RNA酶抑制剂,在进行了持续七天的观察记录后发现。HCV RNA检测结果重复性不佳,RNA酶抑制剂的加入对检测结果的准确性并无太大影响,零下二十摄氏度以及反复冻融操作对PCR检测结果的阳性率也并未产生明显影响,基本可以排除这两种保存条件对HCV RNA血清荧光检测准确性产生影响的可能性。
OBJECTIVE: To study the differences of serum HCV RNA monitoring results under different storage conditions and to analyze the effect of storage conditions. Methods: The collected serum samples were divided into two groups A and B, group A was stored at minus 20 degrees Celsius for seven days, group B was added to the serum samples based on group A RNase inhibitor at minus Twenty degrees Celsius for seven days, compared with the two serum test results. Results: The differences between the two groups of serum samples did not reach statistical significance. The addition of RNase inhibitor did not affect the results of HCV RNA test. CONCLUSIONS: During the experimental study, 40 sera from 20 patients were collected, each of which was stored at minus 20 degrees Celsius. One group was given RNase inhibitor and was observed after seven days of observation. HCV RNA test results were poor repeatability, RNase inhibitor added on the accuracy of the test results did not have much effect, minus 20 degrees Celsius and repeated freeze-thaw operation PCR test results did not have a significant positive rate, Basically, these two storage conditions can be ruled out the possibility of HCV RNA serum fluorescence detection accuracy.