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目的探讨调衡方对S180荷瘤小鼠腹水癌细胞的作用及免疫功能的影响。方法将60只昆明种雄性小鼠随机分为模型组,调衡方大、中、小剂量组(按生药50、25、12.5g/kg),贞芪扶正胶囊组(0.83g/kg),热休克组,将传代培养的S180癌细胞接种于昆明种小鼠腹腔,灌胃给药12d后处死小鼠,分别抽取腹水制片,光镜下观察细胞形态,并采用免疫组织化学法检测腹水CD4、CD8表达及血清白细胞介素-10(IL-10)的活性。结果调衡方中剂量组可见腹水S180癌细胞大量坏死和凋亡,模型组对肿瘤细胞形态无明显影响;调衡方中、大剂量组腹水中CD4、CD8表达均增多(P<0.01),而热休克组则主要是CD8表达升高(P<0.05)。调衡方在25、50g/kg剂量下,对S180荷瘤小鼠外周血IL-10活性水平有抑制作用(P<0.05)。结论调衡方对小鼠腹水S180癌细胞荷瘤小鼠的抑瘤作用机制可能与抑制小鼠外周血IL-10的活性及增强CD4、CD8的表达水平有关,调衡方亦可致S180癌腹水癌细胞坏死和凋亡,机制可能与其提高细胞免疫功能,从而增强机体对肿瘤细胞的免疫杀伤能力有关,从而阻止了肿瘤细胞的侵袭转移。
Objective To investigate the effect of Diaohengfang on S180 tumor-bearing mouse ascites carcinoma cells and immune function. Methods Sixty Kunming male mice were randomly divided into model group and control group, middle and small dosage groups (50,25 and 12.5g / kg for crude drugs), Zhenqi Fuzheng Capsule (0.83g / kg) Heat shock group, the passage of S180 cancer cells were inoculated in the Kunming mice peritoneal cavity after intragastric administration 12d mice were sacrificed, were ascites tablets, observed under light microscope cell morphology, and immunohistochemical detection of ascites CD4, CD8 expression and serum interleukin-10 (IL-10) activity. Results The model group showed no obvious effect on tumor cell morphology. The expression of CD4 and CD8 in ascites of the middle-dose and high-dose groups were increased (P <0.01) However, heat shock group mainly increased CD8 expression (P <0.05). Tiaoheng Recipe can inhibit the level of IL-10 in the peripheral blood of S180 tumor-bearing mice at the dose of 25 and 50g / kg (P <0.05). Conclusion Tiaoheng Recipe has antitumor effects on mouse ascites S180 tumor-bearing mice, which may be related to inhibiting the activity of IL-10 in peripheral blood and enhancing the expression of CD4 and CD8 in mice. Tiaoheng Recipe may also cause S180 carcinoma Ascites cancer cell necrosis and apoptosis, the mechanism may be related to its enhanced cellular immune function, thereby enhancing the body’s ability to immune cells of tumor cells, thereby preventing the invasion and metastasis of tumor cells.