采用ＭＴＴ法检测了人舌鳞状细胞癌ＳＣＣ－２５和ＳＣＣ－９两株细胞对４种临床常用抗癌药物单一用药及联合用药方案的化疗药物敏感性。单一用药的化疗药物敏感性顺序为：顺铂、博莱霉素、氨甲喋呤、５－氟脲嘧啶，以顺铂为主的联合用药化疗方案的抗癌作用明显优于单一用药，并在较低浓度时亦能达到５０％以上的肿瘤生长抑制率。本实验结果对选择临床用药有一定的指导意义。ＭＴＴ法是一种有希望的、值得进一步研究发展的临床肿瘤药敏试验方法。 MTT assay was used to detect the chemosensitivity of human tongue squamous cell carcinoma cell lines SCC-25 and SCC-9 to single-agent and combined-drug regimens for four commonly used anticancer drugs. Single-agent chemotherapeutics have the following susceptibility sequences: cisplatin, bleomycin, methotrexate, 5-fluorouracil, and cisplatin-based combination chemotherapy have significantly better anti-cancer effects than monotherapy, and are lower At the same time, tumor growth inhibition rate of more than 50% can be achieved. The experimental results have a certain guiding significance for the selection of clinical medication. The MTT method is a promising clinical drug susceptibility testing method worthy of further research and development.