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An analytical method based on solid phase extraction was developed and validated for analysis of adefovir in human plasma.Adefovir-d_4 was used as an internal standard and Synergi MAX RP80A(150 mm × 4.6 mm,4 μm) column provided the desired chromatographic separation of compounds followed by detection with mass spectrometry.The method used simple isocratic chromatographic condition and mass spectrometric detection in the positive ionization mode.The calibration curves were linear over the range of 0.50-42.47 ng/mL with the lower limit of quantitation validated at 0.50 ng/mL.Matrix effect was assessed by post-column infusion experiment to monitor phospholipids and postextraction addition experiment was performed.The degree of matrix effect for adefovir was deteimined as 7.5% and ion-enhancement in live different lots of human plasma was 7.1% and had no impact on study samples analysis with 4.5 min run time.The intra- and inter-day precision values were within 7.7% and 7.8%,respectively,for adefovir at the lower limit of quantification level.Validated bioanalytical method was successfully applied to clinical sample analysis.
An analytical method based on solid phase extraction was developed and validated for analysis of adefovir in human plasma. Adefovir-d_4 was used as an internal standard and Synergi MAX RP80A (150 mm × 4.6 mm, 4 μm) column provided the desired chromatographic separation of compounds followed by detection with mass spectrometry. The method used simple isocratic chromatographic condition and mass spectrometric detection in the positive ionization mode. The calibration curves were linear over the range of 0.50-42.47 ng / mL with the lower limit of quantitation validated at 0.50 ng / mL. Matrix effect was assessed by post-column infusion experiment to monitor phospholipids and postextraction addition experiment was performed. degree of matrix effect for adefovir was deteined as 7.5% and ion-enhancement in live different lots of human plasma was 7.1% and had no impact on study samples analysis with 4.5 min run time. The intra- and inter-day precision values were within 7.7% and 7.8% respectively , for adefovir at the lower limit of quantification level. Valued bioanalytical method was successfully applied to clinical sample analysis.