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目的 建立转基因人癌细胞株,寻找基因修饰后人胃腺癌细胞的生物学特性的改变。方法 以逆转录病毒PLXSN为载体,白细胞介素基因(IL—2)为目的基因,人胃腺癌细胞株(MKN—45)为靶标,建立了转基因人胃腺癌细胞株(MKN—45/IL—2)并对该细胞的形态学(光镜和电镜)细胞生长曲线(MTT方法)外源基因的稳定性(PCR方法,G418抗性)和基因表达情况(IL—2分泌量)等生物学特性进行了研究。结果 建立了转基因人胃腺癌细胞株MKN—45/IL—2。外源基因IL—2在该细胞中稳定存在,高效表达。结论 逆转录病毒介导转基因人癌细胞株建立的可行性。目的基因在转基因人癌细胞中稳定存在并高效表达。
Objective To establish a transgenic human cancer cell line and look for changes in the biological characteristics of human gastric adenocarcinoma cells after genetic modification. METHODS: Retroviral PLXSN was used as the vector, interleukin gene (IL-2) as the target gene, and human gastric adenocarcinoma cell line (MKN-45) as the target. The transgenic human gastric adenocarcinoma cell line (MKN-45/IL-) was established. 2) The morphology of the cell (light and electron microscopy) Cell growth curve (MTT method) The stability of foreign genes (PCR method, G418 resistance) and gene expression (IL-2 secretion) and other biology Characteristics were studied. Results The transgenic human gastric adenocarcinoma cell line MKN-45/IL-2 was established. The exogenous gene IL-2 is stably present in the cell and is highly expressed. Conclusion The feasibility of retrovirus-mediated transgenic human cancer cell lines establishment. The target gene is stably present and highly expressed in transgenic human cancer cells.