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采用水煎醇沉法,用正交实验探索银杏叶中黄酮类化合物的最佳提取工艺,并研究复方银杏叶液的镇痛作用。采用煎煮法提取银杏叶药材,醇沉法来纯化,以银杏叶中黄酮含量为指标,选取加水量、煎煮时间、煎煮次数为因素,用正交实验设计方法对银杏叶的提取工艺进行优化,同时在小鼠扭体反应模型上观察其用药后扭体潜伏期和扭体数的变化。银杏叶的最佳提取工艺为14倍加水量,每次煎煮100 min,煎煮3次。复方银杏叶液50、100、200 mg/kg腹腔注射分别显著减少小鼠扭体数,显著延长小鼠扭体潜伏期,并呈一定的剂量依赖性关系。该提取工艺合理可行,与以前文献提取方法比较,减少经济成本,同时袁明复方银杏叶液有明显的镇痛作用。
The decoction of alcohol decoction method, using orthogonal experiment to explore the best extraction of flavonoids in Ginkgo biloba extract, and study the analgesic effect of compound ginkgo leaf liquid. Ginkgo biloba extract by decocting method, alcohol precipitation method to purify the content of flavonoids in Ginkgo biloba leaves as an indicator, select the amount of water, boiling time, boiling times as a factor, using orthogonal experimental design method of extraction of Ginkgo biloba The changes of writhing and writhing were observed after the administration of the drug in writhing reaction model of mice. Ginkgo biloba best extraction process for the 14 times the amount of water, each boiling 100 min, boiling 3 times. The intraperitoneal injection of 50,100,200 mg / kg compound Ginkgo biloba extract significantly reduced the number of writhing in mice and prolonged the writhing period of mice in a dose-dependent manner. The extraction process is reasonable and feasible, compared with the previous literature extraction methods to reduce economic costs, while Yuan Ming Compound Ginkgo biloba fluid has a significant analgesic effect.