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探讨砷对脑星形胶质细胞的毒性作用。以原代培养脑星形胶质细胞(astrocyte,AST)为实验对象,在含砷浓度分别为0、5、10、20、30μmol/L的培养液中培养24h,采用MTT法检测细胞活力,流式细胞仪测定细胞线粒体膜电位的水平,荧光双波长分光光度计法检测细胞内游离钙离子浓度([Ca2+]i)。与对照组相比,AST活力及线粒体膜电位水平随砷暴露浓度的增加而明显下降;[Ca2+]i随砷暴露浓度的增加而显著升高。砷对AST具有明显的毒性作用,在5～30μmol/L的浓度范围内其毒性作用随砷暴露浓度的增加而增强,可引起AST内钙超载,并破坏线粒体功能。
To explore the toxic effects of arsenic on brain astrocytes. Primary cultured astrocytes (AST) were cultured in culture medium containing arsenic at concentrations of 0, 5, 10, 20 and 30 μmol / L for 24 h. Cell viability was measured by MTT assay. The level of mitochondrial membrane potential was measured by flow cytometry, and the intracellular free calcium concentration ([Ca2 +] i) was detected by fluorescence double wavelength spectrophotometer. Compared with the control group, AST activity and mitochondrial membrane potential decreased significantly with the increase of arsenic exposure concentration; [Ca2 +] i increased with the increase of arsenic exposure concentration. Arsenic has a significant toxic effect on AST. The toxic effect of arsenic on AST increased with the increase of arsenic concentration in the concentration range of 5 ~ 30μmol / L, which could cause overload of calcium in the AST and destroy the mitochondrial function.