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目的:探讨外源性磷酸肌酸(PCr)在缺氧缺血性脑损伤中对线粒体功能的影响。方法:将96只七日龄Wistar新生鼠随机分为四组,分别为A假手术组、B生理盐水对照组、C磷酸肌酸小剂量干预组、D磷酸肌酸大剂量干预组,每组24只。除假手术组外其余三组动物制成HIBD模型,并在术前1h分别给予生理盐水、小剂量磷酸肌酸、大剂量磷酸肌酸腹腔注射,24h后取脑,观察脑组织线粒体内三磷酸腺苷(ATP)、还原型谷胱甘肽(GSH)以及丙二醛(MDA)的含量变化。结果:外源性磷酸肌酸干预后脑组织中线粒体内ATP、GSH、MDA含量与盐水对照组相比具有差异性(P<0.05)。结论:外源性磷酸肌酸可以改善线粒体能量代谢,减轻脂质过氧化,保护脑组织。
Objective: To investigate the effect of exogenous creatine phosphate (PCr) on mitochondrial function in hypoxic-ischemic brain damage. Methods: 96 seven-day-old Wistar newborn rats were randomly divided into four groups: A sham operation group, B saline control group, C low dose phosphate creatinine intervention group, D high dose phosphate creatinine intervention group, each group 24 only. Except sham group, the other three groups of animals were made into HIBD model. The rats were given normal saline, low dose of creatine phosphate and high dose of creatine phosphate intraperitoneally 1 h before operation. Brain was taken 24 hours later to observe the changes of mitochondria of adenosine triphosphate ATP), reduced glutathione (GSH) and malondialdehyde (MDA) content. Results: The content of ATP, GSH and MDA in mitochondria of brain tissue after the intervention of exogenous creatine phosphate was different from that of saline control group (P <0.05). Conclusion: Exogenous creatine phosphate can improve mitochondrial energy metabolism, reduce lipid peroxidation and protect brain tissue.