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采用固相合成法将聚集诱导发光分子NI-COOH与GSH响应的多肽序列共价连接, 得到兼具NI探针的聚集诱导发光性能和GSH响应性的多肽探针 (NI-GFFKESSRGD), 可用于GSH的体外检测.在50～600μmol/L的GSH浓度范围内, 多肽探针的荧光强度和GSH浓度呈良好的线性关系, 在该范围内可对未知浓度的GSH样品进行准确定量, 误差小于0.5％.当多肽的浓度进一步增加至2.0％ (w/w) 时, 可形成GSH响应的超分子水凝胶, 其微观形貌上呈现纳米纤维交联的网状结构, 流变性能上具有良好的粘弹特性和剪切稀化特点, 是一类优秀的可注射性材料.多肽探针对成纤维细胞和肿瘤细胞具有良好的细胞相容性, 表明该探针在非入侵性的活体生物分子检测上具有良好的应用前景.“,”Herein we report the syntheses of a GSH-responsive peptide probe NI-GFFKESSRGD which was conjugated with an aggregation-induced-emission dye NI-COOH (AIEgen). GSH reduced the disulfide bond of the peptide and cleaved the hydrophilic moiety RGD, resulting in the self-assembly of peptide and formation of supramolecular hydrogels. When the GSH concentration was in the range of50-600 μmol/L, the fluorescence intensity of peptide was in good linear relationship with the GSH concentration, thereby leading to the accurate measurement of GSH samples with an accuracy of>99. 5％. A further increase of peptide concentration to 2. 0 (w/w)conferred a GSH-responsive supramolecular hydrogel. The hydrogel contained crosslinked network of nanofibers, and displayed excellent viscoelastic and shear-thinning properties, making it an ideal candidate as injectable materials. Finally, these peptide probes showed excellent cytocompatibility to both fibroblast and cancer cells, and could be utilized in noninvasive GSH assays for live cells in future.