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目的了解痂下水肿液(Subeschar tissue fluid,STF)对内皮细胞的损伤作用,进一步论证 STF 是烧伤后一“贮毒库”的概念。方法取烧伤病人痂下水肿液,按20％比例与体外培养的人脐静脉内皮细胞(HUVEC)培养1,12,24h,测定培养液中乳酸脱氢酶(LDH)和6-keto-PGF 1α,观察内皮细胞活力和形态改变,并用健康人血清作对照。结果 STF 与 HUVEC 一同孵育后培养液中LDH 活性和6-keto-PGF 1α含量逐渐升高,并明显高于健康人血清对照组(P<0.01),且 STF 与 HU-VEC 共同孵育24h,内皮细胞活力下降,内皮细胞出现收缩,细胞间间隙增大,部分细胞脱落和坏死,而健康人血清未能引起内皮细胞功能和形态改变。结论 STF 可损伤内皮细胞,在烧伤后内皮细胞的损伤中起重要作用。
Objective To investigate the effect of subeschar tissue fluid (STF) on endothelial cell injury, and to further demonstrate that STF is a post-burn concept. Methods Subcutaneous edematous fluid was collected from burn patients and cultured with human umbilical vein endothelial cells (HUVEC) in the ratio of 20% for 1, 12 and 24 hours respectively. The concentrations of lactate dehydrogenase (LDH) and 6-keto-PGF 1α , Observed endothelial cell viability and morphological changes, and healthy human serum as a control. Results After incubated with HUVEC, the LDH activity and the content of 6-keto-PGF 1α gradually increased in STF-treated HUVEC group and significantly higher than that in healthy human serum control group (P <0.01). STF and HU-VEC incubated for 24 h, Cell viability decreased, endothelial cells contracted, intercellular space increased, part of the cells shedding and necrosis, and healthy human serum failed to cause endothelial cell function and morphological changes. Conclusion STF can damage endothelial cells and play an important role in the injury of endothelial cells after burn injury.