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作者应用免疫荧光试验对阿米巴肝脓肿病人脓液中的阿米巴进行检测。试验标本来自经临床,血清学和肝扫描或超声波确诊的阿米巴肝脓肿病人引流的脓液。对照脓液标本取材于皮下组织和腺体炎性损伤而产生脓肿的病人。肝活检是从8个病人的阿米巴肝脓肿边缘取材。阿米巴抗体系用肝阿米巴脓肿病人血清(间接血凝抗体滴度1:354,294),经分离。提纯后结合异流氰酸荧光素(FITC)。方法是:待检脓液一小滴置于玻片上制成薄涂片,室温凉干;用丙酮固定
The authors used immunofluorescence test for amoeba in patients with amoebic liver abscess pus. Test specimens from clinical, serological and liver scan or ultrasound diagnosis of amoebic abscess drainage of patients with pus. Control pus specimens drawn from subcutaneous tissue and gland inflammatory injury and abscess patients. Liver biopsies were taken from the margins of eight patients with amoebic liver abscesses. Amoeba anti-human liver amoebic abscess patient serum (indirect hemagglutination antibody titer 1: 354,294), after separation. Purification combined with fluorescein isocyanurate (FITC). Method is: to be seized a small drop of pus placed on the slide made of thin smear, dry at room temperature; fixed with acetone