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目的 :实验观察中药合剂AA 3在治疗重症胰腺炎时抑制炎性介质的作用。 方法 :SD大鼠随机分为假手术组(SO组 )、模型组 (SAP组 )和中药治疗组 (AA 3组 ) ,改良式激活剂胰被膜均匀浸润法复制模型。中药合剂AA 3以生大黄、丹参、生山栀为主。用酸度法测定PLA2 的活性 ,用黄嘌呤氧化酶法测定SOD的含量。用硫代巴比妥酸比色法测定MDA的含量。结果 :SAP组大鼠的组织和血清中PLA2 活性、MDA含量均较SO组大鼠升高 (P <0 .0 5 ) ,而SOD含量则低于SO组大鼠 (P <0 .0 5 )。经中药治疗后的AA 3组PLA2 活性、MDA的含量显著降低 ,而SOD的含量上升 ,统计学比较有显著差异。 结论 :中药合剂AA 3具有抑制炎性介质的作用 ,防止SAP的重症化过程 ,并从一个方面解释了中西医结合治疗SAP的机理。
Objective : To observe the effect of Chinese medicine mixture AA 3 in inhibiting inflammatory mediators in the treatment of severe pancreatitis. Methods : SD rats were randomly divided into sham operation group (SO group), model group (SAP group) and Chinese medicine treatment group (AA group 3). The improved activator was uniformly infiltrated with pancreatic capsule to replicate the model. Traditional Chinese medicine mixture AA 3 is mainly made of rhubarb, salvia miltiorrhiza, and raw hawthorn. The activity of PLA2 was measured by the acidity method and the SOD content was measured by the xanthine oxidase method. The MDA content was determined by thiobarbituric acid colorimetry. Results: The PLA2 activity and MDA content in the tissue and serum of the SAP rats were higher than those in the SO rats (P < 0.05), but the SOD content was lower than that in the SO rats (P <0. ). After treatment with traditional Chinese medicine, PLA2 activity and MDA content in AA 3 group were significantly decreased, while SOD content was increased, and there was a statistically significant difference. Conclusion: AA 3 of traditional Chinese medicine mixture has the function of inhibiting inflammatory mediators and preventing the severe process of SAP. It also explains the mechanism of the treatment of SAP by combination of TCM and western medicine.