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本实验获得了白鲢鱼胰岛素的单晶体并收集了2.8A分辨率的强度数据.确定晶体所属空间群为 P2_12_12_1,晶胞参数是a=96.73(8)A,b=57.74(7)A,c=53.88(9)A.每个不对称单位含有6个胰岛素分子.用旋转函数法揭示出非晶体学三重轴和二重轴的取向,并说明了白鲢鱼胰岛素具有与三方二锌猪胰岛素相似的空间结构和聚合状态.本文讨论了一级结构的变化对胰岛素的结晶行为和堆积方式的影响。
In this experiment, the single crystals of silver carp insulin were collected and the intensity data of 2.8A resolution was collected. The space group of crystal belongs to P2_12_12_1, the unit cell parameters are a = 96.73 (8) A, b = 57.74 (7) A, c = 53.88 (9) A. Each of the asymmetric units contains six insulin molecules The rotation function method reveals the orientation of the non-crystallographic triplet and double axes and illustrates that the silver carp insulin has the same insulin activity as the tribasin Zinc porcine insulin Similar spatial structure and aggregation state.This paper discusses the effect of primary structure changes on the crystallization behavior and accumulation of insulin.