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目的研究石墨炉原子吸收光谱法测定纳米银烧伤贴在新西兰白兔实验中银检测及在各组织中的分布情况。方法研究仪器条件、基体改进剂对测定的影响,选择测定方法不同组织最理想条件,并测定兔组织中银的含量及日间、日内和加标回收试验,作方法验证。结果测定血浆中银的线性范围为0.15~10.00μg/L(R2=0.998),加标回收率为89.4%~108.2%,RSD<2.9%(n=11),方法检出限为0.08μg/L。测定脏器中银的线性范围为0.24~10.00μg/L(R2=0.996),加标回收率为85.9%~110.4%,RSD<3.6%(n=11),方法检出限为0.17μg/L。结论该方法操作简便,检出限低,定量准确可靠,重现性好,可满足生物样品中低含量银的测定需要。通过对给药组与基质对照组比较结果表明,给药时间越长,纳米银颗粒含量越多,而银除了在脾脏和脑组织中含量有明显蓄积外,血液和其他组织无明显变化。
Objective To study the silver detection and its distribution in different tissues of New Zealand white rabbits by graphite furnace atomic absorption spectrometry. Methods The instrument conditions, the influence of the matrix modifier on the determination, the optimal conditions of different tissues were determined, and the content of silver in the tissues of the rabbits was determined. The daily, intraday and spike recovery tests were conducted for validation. Results The linear range of determination of silver in plasma was 0.15-10.00μg / L (R2 = 0.998). The spiked recoveries were 89.4% -108.2% with RSD <2.9% (n = 11) . The linear range was 0.24-10.00μg / L (R2 = 0.996). The spiked recoveries were 85.9% -110.4%, RSD <3.6% (n = 11) .The detection limits were 0.17μg / L . Conclusion The method is simple, low detection limit, accurate and reliable quantitative, reproducible, and can meet the needs of determination of low levels of silver in biological samples. The results of comparison between the administration group and the matrix control group showed that the longer the administration time, the more the content of nano-silver particles, and the addition of silver in the spleen and brain tissue in addition to the significant accumulation of blood and other tissues without significant change.