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观察了１８～２８周龄胎儿脾单个核细胞（ＦＳＭＣ）在体外对ＯＫＴ３＋ｒｈＩＬ－２联合刺激的反应性，结果发现：ＯＫＴ３单独能活化ＦＳＭＣ，最适浓度ＯＫＴ３与ｒｈＩＬ－２联合对ＦＳＭＣ有强协同刺激作用。ＯＫＴ３刺激ＦＳＭＣ后明显促进ＩＬ－２Ｒ表达，表明ＯＫＴ３对ＦＳＭＣ的括化作用与ＩＬ－２／ＩＬ－２Ｒ途径相关。ＯＫＴ３＋ｒｈＩＬ－２协同诱导的ＦＳＭｃ能产生ＮＫ和ＬＡＫ活性，且ＬＭ活住较单用ｒｈＩＬ－２诱导者强，间接免疫荧光染色ＦＡＣＳ分析显示，ＯＫＴ３＋ｒｈｌＬ－２协同激活的ＦＳＭＣ主要是ＣＤ８￣＋Ｔ细胞。结果表明ＦＳＭＣ与成人ＰＢＭＣ－样能被ＯＫＴ３活化。
OKT3 + rhIL-2 co-stimulated reactivity of fetal spleen mononuclear cells (FSMCs) from 18 to 28 weeks of gestation was observed. OKT3 alone can activate FSMC, and the combination of OKT3 and rhIL-2 at the optimal concentration has strong synergistic effect on FSMC Stimulation. OKT3 significantly stimulated the expression of IL-2R after FSMC, indicating that OKT3 on the FSMC’s involvement and IL-2 / IL-2R pathway. OKT3 + rhIL-2 synergistically induced NK and LAK activity, and LM live was stronger than rhIL-2 alone. FACS analysis showed that the synergistic activation of OKT3 + rhlL-2 was mainly CD8 + T cells. The results show that FSMC and adult PBMC-like can be OKT3 activation.