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[目的]观察人类甲状腺癌中HER 2/neu癌蛋白和mRNA的表达及其关系。[方法]应用免疫组化和同位素点杂交技术对新鲜冰冻甲状腺组织HER 2/neu癌蛋白和mRNA的表达进行研究。[结果]21例甲状腺乳头状癌中14例HER 2/neu癌蛋白表达阳性 ,而其他类型肿瘤和非肿瘤组织均未见HER 2/neu表达。HER 2/neumRNA表达研究显示 ,乳头状癌和淋巴结转移灶中mRNA的表达水平明显高于其他肿瘤和非肿瘤组织。本研究还证明石蜡包埋的乳头状癌标本中HER 2/neu癌蛋白表达率明显低于新鲜标本。[结论]HER 2/neu蛋白的表达是甲状腺乳头状癌的一个特征性改变 ,HER 2/neu蛋白的表达与mRNA的表达相一致 ,HER 2/neu蛋白检测的稳定性取决于组织标本的质量
[Objective] To observe the expression and relationship of HER 2/neu oncoprotein and mRNA in human thyroid carcinoma. [Methods] The expression of HER 2/neu oncoprotein and mRNA in fresh frozen thyroid tissue was studied by immunohistochemistry and isotope point hybridization. [Results] The expression of HER 2/neu oncoprotein was positive in 14 cases of papillary thyroid carcinoma, while no expression of HER 2/neu was found in other types of tumors and non-tumor tissues. HER 2/neumRNA expression studies have shown that mRNA expression levels in papillary and lymph node metastases are significantly higher than in other tumors and non-tumor tissues. This study also demonstrated that the expression rate of HER 2/neu oncoprotein in paraffin-embedded papillary carcinoma specimens was significantly lower than that of fresh specimens. [Conclusion] The expression of HER 2/neu protein is a characteristic change of papillary thyroid carcinoma. The expression of HER 2/neu protein is consistent with the expression of mRNA. The stability of HER 2/neu protein detection depends on the quality of tissue specimens.