论文部分内容阅读
目的:探讨不同温度下对小鼠成骨细胞MC3T3-E1的增殖以及OPG/RANKL表达水平的影响。方法:1.以小鼠成骨细胞MC3T3-E1为体外实验模型,MTT法检检测细胞的增殖情况。2.RT-PCR方法检测MC3T3-E1OPG/RANKL mRNA的表达水平。结果:设定对照组为37℃,高于对照组(38℃-39℃-40℃-41℃-42℃)分别作用于MC3T3-E1细胞1小时/天,连续1周,可刺激细胞增殖,OD值显著增加(P<0.05)。同时可增加OPG mRNA表达,降低RANKL mRNA表达,呈温度梯度依赖性。结论:热刺激促进MC3T3-E1细胞增殖,同时通过调节OPG/RANKL mRNA的表达,直接促进骨形成,抑制骨吸收。
Objective: To investigate the effect of different temperature on the proliferation of osteoblast MC3T3-E1 and the expression of OPG / RANKL. Methods: 1. The mouse osteoblast MC3T3-E1 experimental model in vitro, MTT assay was measured cell proliferation. The expression of MC3T3-E1OPG / RANKL mRNA was detected by RT-PCR. Results: The control group was set at 37 ℃, which was higher than the control group (38 ℃ -39 ℃ -40 ℃ -41 ℃ -42 ℃), respectively, for 1 hour / day for 1 week, which could stimulate the proliferation of MC3T3-E1 cells , OD value increased significantly (P <0.05). At the same time, it can increase OPG mRNA expression and decrease RANKL mRNA expression in a temperature gradient dependent manner. Conclusion: Thermal stimulation can promote the proliferation of MC3T3-E1 cells. At the same time, by regulating the expression of OPG / RANKL mRNA, it can directly promote the bone formation and inhibit the bone resorption.