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呋喃西林溶液常用其1:5000浓度,含量测定有比色法:氧化还原法等,比色法误差较大,氧化还原法操作不够简便。我们应用紫外分光光度法测定其含量,简便、迅速、准确,现报告如下。 (一)主要仪器与药品 751G分光光度计、呋喃西林(沈阳红卫制药厂产)、生理盐水(自制)。 (二)方法与结果 1.呋喃西林紫外光谱:精确称取20mg呋喃西林,用容量瓶加生理盐水配成100ml,即200μg/ml标准液,取此液1ml,精确加入生理盐水19ml,混匀,即为10μg/ml标照液,用此液在200～400nm范围内测取吸收度,得呋喃西林紫外吸收光谱,其λmax375nm(图1)。
Nitrofuranto commonly used solution of its 1: 5000 concentration, determination of colorimetric method: redox method, colorimetric error, redox method is not easy enough. We use UV spectrophotometry determination of its content, simple, rapid and accurate, are as follows. (A) of the main instruments and medicines 751G spectrophotometer, nitrofurazone (Shenyang Hongwei Pharmaceutical Factory), saline (self-made). (B) methods and results 1. Nitrofurazone ultraviolet spectroscopy: accurately weighed 20mg nitrofurazone, with a volumetric flask plus normal saline dubbed 100ml, that is, 200μg / ml standard solution, take this solution 1ml, accurately added 19ml of normal saline, mix, that is 10μg / ml standard solution, using this solution in the range of 200 ~ 400nm absorbance was measured to give nitrofurazone ultraviolet absorption spectrum, its λmax375nm (Figure 1).