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以星斑川鲽(Platichthys Stellatus;初始体质量为75.6±0.18g)为研究对象,以新型蛋白源(大豆浓缩蛋白、脱酚棉籽蛋白、喷雾干燥血球蛋白粉等)为原料,通过调节饲料氨基酸水平,配制出5种等氮等能的饲料D0、D17、D35、D52和D69,其中D0(添加58%脱脂鱼粉)为对照组,其余各组分别替代17%、35%、52%和69%的鱼粉。试验共分5组,每组设3个平行,试验期60d。结果表明,当新型蛋白源替代鱼粉量的35%,胃蛋白酶活力开始下降为36.87U/mg.min,D35、D52和D69组无显著性差异(P>0.05),显著小于D0和D17组(P<0.05);当替代鱼粉量的69%时,肠道蛋白酶活力显著降低,小于其他4组(P<0.05);且胃蛋白酶活力显著高于肠道蛋白酶活力(P<0.05)。蛋白质消化率和干物质消化率在前三组间无显著差异(P>0.05),显著高于D52和D69组(P<0.05),D69组的平均增重率显著低于其他4组(P<0.05),且饲料系数显著高于前四组(P<0.05)。试验结果表明,用新型蛋白源替代饲料中35%的鱼粉是可行的。
Using Platichthys stellatus (initial body weight of 75.6 ± 0.18g) as the research object, a novel protein source (soy protein concentrate, phenol-denatured cottonseed protein, spray-dried hemoglobin powder, etc.) (D0) (58% defatted fish meal) were used as the control group, while the other groups were respectively replaced by 17%, 35%, 52% and 60% 69% of fish meal. The experiment is divided into 5 groups, each set 3 parallel, the test period 60d. The results showed that pepsin activity began to decrease to 36.87U / mg.min when the new protein source was replaced by 35% fish meal, but no significant difference between D35, D52 and D69 (P> 0.05) P <0.05). When 69% fish meal was replaced by fish meal, the protease activity of intestinal mucosa decreased significantly, which was significantly lower than that of the other four groups (P <0.05). The activity of pepsin was significantly higher than that of intestinal protease (P <0.05). The protein digestibility and dry matter digestibility were not significantly different among the three groups (P> 0.05), but significantly higher than those in D52 and D69 (P <0.05). The average weight gain of D69 group was significantly lower than that of the other four groups <0.05), and the feed coefficient was significantly higher than the first four groups (P <0.05). The experimental results show that it is feasible to replace 35% of the fishmeal in the feed with a new protein source.