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目的：研究SD雌性大鼠骨髓单核细胞（BMM）、雌激素受体阳性的骨髓单核细胞[ER（＋）BMM]率与大鼠心脏血雌激素E2在抗骨质疏松中的作用。方法：选择3个月龄雌性SD大鼠40只，随机分成正常对照组、假手术组、去势组、去势后雌激素替代治疗组，每组10只，同样条件下饲养，3个月后断头处死检测。（1）用SP染色显示骨髓单核细胞ER；（2）用非特异性酯酶（α－NAE）法计数单核细胞；（3）用放免法测定大鼠心脏血E2；（4）取SD大鼠股骨干骺端作病理切片检查。结果：去势组比其他三组BMM数明显升高（P＜0．01），而ER（＋）BMM率和E2明显下降（P＜0．01）。病理切片显示明显骨质疏松形成，而去势后给予信美力的大鼠各参数与正常对照组和假手术组差异不明显（P＞0．05）。提示：雌激素可能影响骨髓单核细胞数，与骨质疏松的发生存在一定内在联系。
OBJECTIVE: To investigate the effects of estrogen receptor-positive myelomonocytic [ER (+) BMM] ratio and rat cardiac blood estrogen E2 on anti-osteoporosis in SD female rats. Methods: Forty female SD rats of 3 months old were randomly divided into normal control group, sham operation group, castrated group and castrated estrogen replacement group, 10 rats in each group. The animals were housed under the same conditions for 3 months Post-decapitation test. (1) SP staining of bone marrow mononuclear cells ER; (2) nonspecific esterase (α-NAE) counting mononuclear cells; (3) radioimmunoassay rat heart blood E2; (4) Pathological examination of the femoral metaphysis in rats. Results: The number of BMM in ovariectomized group was significantly higher than that in other three groups (P <0.01), but the rate of ER (+) BMM and E2 decreased significantly (P <0.01). The pathological sections showed obvious osteoporosis. However, there was no significant difference in the parameters of the rats given Xinmeili after castration compared with the normal control group and the sham operation group (P> 0.05). Tip: Estrogen may affect the number of bone marrow mononuclear cells, and the occurrence of osteoporosis there is some intrinsic link.