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26个苹果属植物种类受苹果褪绿叶斑病毒(CLSV)侵染后,其过氧化物酶活性升高,嫁接后95天内出现1或2个增酶高峰.同工酶的谱带也增多,特别是B 区变化更为明显,但不同种类反应不一,楸子、酸苹果等对CLSV 抗性强的种类出现2个增酶高峰,且表现时间早,峰值低,同工酶出现2次特异性谱带;冬白果等对CLSV 敏感的种类则只有1个增酶高峰,表现时间迟,峰值高,只出现一次特异性谱带。这种过氧化物酶活性及其同工酶谱的变化可作为鉴定苹果属植物对CLSV 的抗性的生化指标.
After 26 apple species were infected by apple chlorosis leaf spot virus (CLSV), their peroxidase activity increased, and one or two peaks of enzyme increased within 95 days after grafting.The isoenzyme bands also increased, In particular, the change in area B was more obvious, but different types reacted differently. Two kinds of enzyme-resistant peak appeared in CLSV-resistant species such as Catalpa bungei and apple, and their expression time was earlier and the peak value was low. Isoenzymes appeared twice Specific bands; Dongbai and other CLSV-sensitive species only one peak of the enzyme, the performance of late, high peak, only a single band. This change in peroxidase activity and its isoenzyme profile can be used as a biochemical indicator of the resistance of apple plants to CLSV.