微透析是一种活体的取样技术,在皮肤药理学的研究中应用广泛。微透析被用于外用制剂的生物等效性、制剂的透皮吸收、监测皮肤炎症介质、皮肤组织的内分泌、中药经皮代谢等相关研究。近年来,微透析向联用各种分析检测技术的方向发展,如联用超高效液相色谱、液质联用、酶标记免疫吸附测定等。然而,该技术本身也存在一些缺点,如油水分配系数较大药物的测定、低含量药物的测定、探针植入重现性等问题。文中综述了该技术的原理、优点、缺点及在皮肤药理学研究中的应用进展。 Microdialysis is a living sampling technique that is widely used in the study of dermatopharmacology. Microdialysis is used for bioequivalence of topical preparations, transdermal absorption of preparations, monitoring of inflammatory mediators of the skin, endocrine of skin tissues, and percutaneous metabolism of Chinese herbs. In recent years, microdialysis to the development of a variety of analytical detection techniques, such as combined with ultra performance liquid chromatography, LC / MS, enzyme-linked immunosorbent assay. However, the technology itself has some shortcomings, such as the determination of drugs with large oil and water partition coefficient, the determination of drugs with low content and the reproducibility of probe implantation. The paper summarizes the principle, advantages and disadvantages of this technique and its application in skin pharmacology.